Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Human "Do-It-Yourself" Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human "Do-It-Yourself" Positive Selection Kit II is designed to select any human cell type of interest with your own mouse IgG1 monoclonal antibody. Cells that express the surface antigen that is recognized by the mouse IgG1 monoclonal antibody are targeted for magnetic separation using EasySep™ Dextran RapidSpheres™ and the EasySep™ Magnet. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding.This product replaces the EasySep™ Human "Do-It-Yourself" Selection Kit (Catalog #18099).
STEMCELL Technologies RosetteSep™ HLA B Cell Enrichment Cocktail, For processing: 1000 mL whole blood processed
Immunodensity isolation of untouched B cells for HLA analysis
STEMCELL Technologies RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail is designed to pre-enrich progenitor cells by removing lineage-specific cells from whole bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD11b, CD14, CD16, CD19, CD56, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The pre-enriched progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RosetteSep™ Human Cord Blood Debulking Cocktail, For processing: 40 mL cord blood processed
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II, Size: 1 x 10^9 cells
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit II is designed to isolate naïve CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit replaces the EasySep™ Human Naïve CD4+ T Cell Enrichment Kit (Catalog #19155) and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
STEMCELL Technologies EasySep™ Direct Human PBMC Isolation Kit RoboSep™, For processing: 100 mL whole blood processed
The EasySep™ Direct Human PBMC Isolation Kit is designed to isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis products by negative selection. Red blood cells (RBCs), platelets, and unwanted cells are targeted for removal with antibody complexes and magnetic particles and separated using an EasySep™ magnet. Untouched PBMCs are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
STEMCELL Technologies RosetteSep™ Human CD45 Depletion Cocktail, For processing: 40 mL whole blood processed
The RosetteSep™ Human CD45 Depletion Cocktail is designed to enrich epithelial circulating tumor cells (CTCs) from whole blood by depleting CD45+ cells. Unwanted cells are targeted for depletion with Tetrameric Antibody Complexes recognizing CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified epithelial tumor cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit is designed to isolate CD3+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T cells and NKT cells.This product replaces the EasySep™ Human Whole Blood CD3 Positive Selection Kit (Catalog #18081) and EasySep™ HLA Whole Blood CD3 Positive Selection Kit (Catalog #18081HLA), for even faster cell isolations.
STEMCELL Technologies RoboSep™ HLA T Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ HLA T Cell Enrichment Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-T cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
STEMCELL Technologies RoboSep™ Human CD14 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD14 Positive Selection Kit II is designed to isolate highly purified CD14+ cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs). Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and macrophages and weakly on granulocytes. It is also expressed on most tissue macrophages.This product replaces the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058), for even faster cell isolations and is compatible with all EasySep™ magnets.
American Research Products Inc Fish Glutathione PeroXidase G
Fish Glutathione PeroXidase (GSH-PX) ELISA kit from CUSABIO Detection Range 375 U/mL-6000 U/mL Sensitivity 187 5 U/mL Sample serum plasma tissue homogenates Method Competitive
Cayman Chemical GlutathIon PeroxIdase asy 96
GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity indirectly by a coupled reaction with GR. Oxidized GSSG, produced upon reduction of an organic hydroperoxide by GPX, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the GPX activity in the sample. Cayman’s GPX Assay can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
Gold Biotechnology Inc NDSB-201 (Non-detergent sulfobetaine) 100 g
NDSB-201 is a protein specific additive that can be used to help solubilize and stabilize proteins at non-denaturing concentrations while preventing protein aggregation.
Cell Signaling Technology SimpleChIP(R) Human LENG8 5' UTR Primers 500 ul
SimpleChIP(R) Human LENG8 5' UTR Primers 500 ul